Digestion Testing with PST/MSP

Enzyme Reaction Testing with Selected Enzymes and Bead Clean

Date Performed: March 18th, 2021 Samples used: 352, 369, 431, 439, 487, 785, 831, 865 (randomly selected)

  1. Aliquoted samples following guidance sheet (below)
  2. Followed protocol for 1X bead clean, resuspended in 70ul 10mM tris HCL
  3. While beads were on orbital, made master mix of enzyme solution
  4. After bead clean procedure, added 10ul master mix to each samples
  5. Ran 8 strip tube of reactions on 12 hour digest in thermocylcer

Infographic of volumes

NOTE: NEVER RUN ON TAPESTATION

Written on March 18, 2021