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DNA(113) • Horseshoe crab(113) • PCR(2) • blue pippin(2) • ddRAD(4) • size selection(3) • troubleshooting(12)

DNA 113

1x Bead Cleans, Digestions, 1.5X Bead Clean and Post Digest Quant for Plate A • August 31, 2021
Additional 4 cycles PCR for all pools • February 24, 2022
Annealing Adapters and Adapter Ligation • June 29, 2021
Bead Cleans and Digestions for Plates A, B, C • April 15, 2021
Bead Cleans and Plate Reader Quantification • June 22, 2021
Bead Testing • May 6, 2021
Blue Pippin Pools 3,4,5 • January 23, 2022
Blue Pippin Size Selection • July 21, 2021
Comparison Run of Qubit and Plate Reader • August 19, 2021
DNA Extraction, Digestion on Plate C, Plate Reader on Plate B • November 3, 2021
Digest Prep - First Bead Clean for Enzyme Testing • January 27, 2021
Digestion (Testing) with Plate B • September 2, 2021
Digestion Testing • May 19, 2021
Digestion Testing • May 16, 2021
Digestion Testing • January 28, 2021
Digestion Testing with 250ng DNA • March 23, 2021
Digestion Testing with PST/MSP • March 18, 2021
Dilution Testing • May 11, 2021
First Half DNA Extraction • March 9, 2022
First Half DNA Extraction • February 16, 2022
Gel Images with Average DNA ng/ul for all 2020 Extractions • December 7, 2020
Gel Images with Average DNA ng/ul for all 2021 Extractions • September 2, 2021
Gel for QAQC Pools 1 and 2 (Tape Station Out of Service) • January 22, 2022
Horseshoe Crab Agarose (Re)Gel Results for 464 and 769 • January 21, 2021
Horseshoe Crab Agarose Gel Protocol • September 15, 2020
Horseshoe Crab Agarose Gel Results • December 3, 2020
Horseshoe Crab Agarose Gel Results • December 1, 2020
Horseshoe Crab Agarose Gel Results • November 24, 2020
Horseshoe Crab Agarose Gel Results • November 14, 2020
Horseshoe Crab Agarose Gel Results • November 12, 2020
Horseshoe Crab Agarose Gel Results • November 5, 2020
Horseshoe Crab Agarose Gel Results • October 29, 2020
Horseshoe Crab Agarose Gel Results • October 15, 2020
Horseshoe Crab Agarose Gel Results • October 8, 2020
Horseshoe Crab Agarose Gel Results • September 16, 2020
Horseshoe Crab Agarose Gel Results • September 12, 2020
Horseshoe Crab Agarose Gel Results from Re-Extraction • January 5, 2021
Horseshoe Crab Agarose Gel Results from Re-Extraction • December 29, 2020
Horseshoe Crab Agarose Gel Results from Re-Extraction • December 22, 2020
Horseshoe Crab DNA (re)Extraction and Quantification • January 11, 2021
Horseshoe Crab DNA (re)Extraction and Quantification • December 29, 2020
Horseshoe Crab DNA (re)Extraction and Quantification • December 22, 2020
Horseshoe Crab DNA (re)Extraction and Quantification • December 10, 2020
Horseshoe Crab DNA Extraction • October 22, 2020
Horseshoe Crab DNA Extraction • October 20, 2020
Horseshoe Crab DNA Extraction • October 13, 2020
Horseshoe Crab DNA Extraction • October 8, 2020
Horseshoe Crab DNA Extraction • October 6, 2020
Horseshoe Crab DNA Extraction • September 11, 2020
Horseshoe Crab DNA Extraction • September 2, 2020
Horseshoe Crab DNA Extraction • August 27, 2020
Horseshoe Crab DNA Extraction • August 21, 2020
Horseshoe Crab DNA Extraction - Version 2 • September 12, 2020
Horseshoe Crab DNA Extraction - Version 3 • September 11, 2020
Horseshoe Crab DNA Extraction and Quantification • December 1, 2020
Horseshoe Crab DNA Extraction and Quantification • November 24, 2020
Horseshoe Crab DNA Extraction and Quantification • November 19, 2020
Horseshoe Crab DNA Extraction and Quantification • November 17, 2020
Horseshoe Crab DNA Extraction and Quantification • November 14, 2020
Horseshoe Crab DNA Extraction and Quantification • November 12, 2020
Horseshoe Crab DNA Extraction and Quantification • November 10, 2020
Horseshoe Crab DNA Extraction and Quantification • November 5, 2020
Horseshoe Crab DNA Extraction and Quantification • November 3, 2020
Horseshoe Crab DNA Extraction and Quantification 2021 Samples • July 25, 2021
Horseshoe Crab DNA Quantification • December 3, 2020
Horseshoe Crab DNA Quantification • October 22, 2020
Horseshoe Crab DNA Quantification • October 20, 2020
Horseshoe Crab DNA Quantification • October 13, 2020
Horseshoe Crab DNA Quantification • October 8, 2020
Horseshoe Crab DNA Quantification • October 6, 2020
Horseshoe Crab DNA Quantification • September 12, 2020
Horseshoe Crab DNA Quantification • September 11, 2020
Horseshoe Crab DNA Quantification • September 11, 2020
Horseshoe Crab DNA Quantification • September 2, 2020
Horseshoe Crab DNA Third Extraction Attempt 464, 769 • January 26, 2021
Ligation Efficiency Test Plate A, Pool Plate B Pool 3 • December 23, 2021
Ligation Efficiency Test, Adapter Ligation, Pool 2 Bead Clean • December 22, 2021
Ligation Efficiency Testing • August 3, 2021
Ligation Efficiency Testing • July 7, 2021
Ligation Test Plates B and C, Bead Clean Plate A • December 1, 2021
Mailing 5 Libraries • March 14, 2022
PCR Index Addition, 1.5X Bead Clean, Post Quibit Size Selected Pools 1 and 2 • January 19, 2022
PCR and Index Addition Pool 1, 1.5X clean pools 1,3,4,5, qubit, gel • February 1, 2022
PCR, Index Addition, 1X Bead Clean, Qubit • July 23, 2021
Plate A 1X Bead Clean and Digestion • November 30, 2021
Plate A Dilution for Ligation • December 19, 2021
Plate A and C Dilutions • November 17, 2021
Plate Aliquots • August 27, 2021
Plate Aliquots • June 3, 2021
Plate Aliquots • April 6, 2021
Plate B 100ng DNA aliquot for adapter ligation, 1.5X bead clean plate C, moving plate A samples • November 9, 2021
Plate B Row A Dilution and Adapter Addition, Plate Reader Plate A • December 18, 2021
Plate B Split, Bead Clean, Plate Read • April 29, 2021
Plate B and C Adapater Ligations • November 27, 2021
Plate Reader - Post 1.5X bead clean plate C and plate A • November 9, 2021
Plate Reader - Raw DNA for Plate A on Plate Reader • November 14, 2021
Pool 1 Blue Pippin Size Selection • January 8, 2022
Pool 2 Blue Pippin Size Selection, QC Pool 1 Size Selected Sample • January 12, 2022
Pool Plate A Pool 1 • December 29, 2021
Pooling Plate C - Pools 4 and 5 • December 16, 2021
Post Digestion Bead Clean • February 2, 2021
Post Digestion Bead Clean and Plate Read • April 27, 2021
Re-run Pool 1 Blue Pippin, Pools 3,4,5 PCR and Index Addition • January 26, 2022
Sample Pooling • August 5, 2021
Sample Pooling • July 8, 2021
Second Attempt Adapter Ligation Dilutions • July 27, 2021
Sorted through all plates and freezer and bead cleaned plate C • November 2, 2021
Summary of all Plate Reader, Qubit, and Tape Station Tests • October 22, 2021
Tape Station Procedure for Digested Samples • February 4, 2021
Tape Station Procedure for Digested Samples (250ng) • March 25, 2021
Tape Station Results Samples 359, 854, 858 • March 30, 2021
Tape Station Results Samples 401, 759, 419 • February 25, 2021
Trouble Shooting with Jon • August 17, 2021

Horseshoe crab 113

1x Bead Cleans, Digestions, 1.5X Bead Clean and Post Digest Quant for Plate A • August 31, 2021
Additional 4 cycles PCR for all pools • February 24, 2022
Annealing Adapters and Adapter Ligation • June 29, 2021
Bead Cleans and Digestions for Plates A, B, C • April 15, 2021
Bead Cleans and Plate Reader Quantification • June 22, 2021
Bead Testing • May 6, 2021
Blue Pippin Pools 3,4,5 • January 23, 2022
Blue Pippin Size Selection • July 21, 2021
Comparison Run of Qubit and Plate Reader • August 19, 2021
DNA Extraction, Digestion on Plate C, Plate Reader on Plate B • November 3, 2021
Digest Prep - First Bead Clean for Enzyme Testing • January 27, 2021
Digestion (Testing) with Plate B • September 2, 2021
Digestion Testing • May 19, 2021
Digestion Testing • May 16, 2021
Digestion Testing • January 28, 2021
Digestion Testing with 250ng DNA • March 23, 2021
Digestion Testing with PST/MSP • March 18, 2021
Dilution Testing • May 11, 2021
First Half DNA Extraction • March 9, 2022
First Half DNA Extraction • February 16, 2022
Gel Images with Average DNA ng/ul for all 2020 Extractions • December 7, 2020
Gel Images with Average DNA ng/ul for all 2021 Extractions • September 2, 2021
Gel for QAQC Pools 1 and 2 (Tape Station Out of Service) • January 22, 2022
Horseshoe Crab Agarose (Re)Gel Results for 464 and 769 • January 21, 2021
Horseshoe Crab Agarose Gel Protocol • September 15, 2020
Horseshoe Crab Agarose Gel Results • December 3, 2020
Horseshoe Crab Agarose Gel Results • December 1, 2020
Horseshoe Crab Agarose Gel Results • November 24, 2020
Horseshoe Crab Agarose Gel Results • November 14, 2020
Horseshoe Crab Agarose Gel Results • November 12, 2020
Horseshoe Crab Agarose Gel Results • November 5, 2020
Horseshoe Crab Agarose Gel Results • October 29, 2020
Horseshoe Crab Agarose Gel Results • October 15, 2020
Horseshoe Crab Agarose Gel Results • October 8, 2020
Horseshoe Crab Agarose Gel Results • September 16, 2020
Horseshoe Crab Agarose Gel Results • September 12, 2020
Horseshoe Crab Agarose Gel Results from Re-Extraction • January 5, 2021
Horseshoe Crab Agarose Gel Results from Re-Extraction • December 29, 2020
Horseshoe Crab Agarose Gel Results from Re-Extraction • December 22, 2020
Horseshoe Crab DNA (re)Extraction and Quantification • January 11, 2021
Horseshoe Crab DNA (re)Extraction and Quantification • December 29, 2020
Horseshoe Crab DNA (re)Extraction and Quantification • December 22, 2020
Horseshoe Crab DNA (re)Extraction and Quantification • December 10, 2020
Horseshoe Crab DNA Extraction • October 22, 2020
Horseshoe Crab DNA Extraction • October 20, 2020
Horseshoe Crab DNA Extraction • October 13, 2020
Horseshoe Crab DNA Extraction • October 8, 2020
Horseshoe Crab DNA Extraction • October 6, 2020
Horseshoe Crab DNA Extraction • September 11, 2020
Horseshoe Crab DNA Extraction • September 2, 2020
Horseshoe Crab DNA Extraction • August 27, 2020
Horseshoe Crab DNA Extraction • August 21, 2020
Horseshoe Crab DNA Extraction - Version 2 • September 12, 2020
Horseshoe Crab DNA Extraction - Version 3 • September 11, 2020
Horseshoe Crab DNA Extraction and Quantification • December 1, 2020
Horseshoe Crab DNA Extraction and Quantification • November 24, 2020
Horseshoe Crab DNA Extraction and Quantification • November 19, 2020
Horseshoe Crab DNA Extraction and Quantification • November 17, 2020
Horseshoe Crab DNA Extraction and Quantification • November 14, 2020
Horseshoe Crab DNA Extraction and Quantification • November 12, 2020
Horseshoe Crab DNA Extraction and Quantification • November 10, 2020
Horseshoe Crab DNA Extraction and Quantification • November 5, 2020
Horseshoe Crab DNA Extraction and Quantification • November 3, 2020
Horseshoe Crab DNA Extraction and Quantification 2021 Samples • July 25, 2021
Horseshoe Crab DNA Quantification • December 3, 2020
Horseshoe Crab DNA Quantification • October 22, 2020
Horseshoe Crab DNA Quantification • October 20, 2020
Horseshoe Crab DNA Quantification • October 13, 2020
Horseshoe Crab DNA Quantification • October 8, 2020
Horseshoe Crab DNA Quantification • October 6, 2020
Horseshoe Crab DNA Quantification • September 12, 2020
Horseshoe Crab DNA Quantification • September 11, 2020
Horseshoe Crab DNA Quantification • September 11, 2020
Horseshoe Crab DNA Quantification • September 2, 2020
Horseshoe Crab DNA Third Extraction Attempt 464, 769 • January 26, 2021
Ligation Efficiency Test Plate A, Pool Plate B Pool 3 • December 23, 2021
Ligation Efficiency Test, Adapter Ligation, Pool 2 Bead Clean • December 22, 2021
Ligation Efficiency Testing • August 3, 2021
Ligation Efficiency Testing • July 7, 2021
Ligation Test Plates B and C, Bead Clean Plate A • December 1, 2021
Mailing 5 Libraries • March 14, 2022
PCR Index Addition, 1.5X Bead Clean, Post Quibit Size Selected Pools 1 and 2 • January 19, 2022
PCR and Index Addition Pool 1, 1.5X clean pools 1,3,4,5, qubit, gel • February 1, 2022
PCR, Index Addition, 1X Bead Clean, Qubit • July 23, 2021
Plate A 1X Bead Clean and Digestion • November 30, 2021
Plate A Dilution for Ligation • December 19, 2021
Plate A and C Dilutions • November 17, 2021
Plate Aliquots • August 27, 2021
Plate Aliquots • June 3, 2021
Plate Aliquots • April 6, 2021
Plate B 100ng DNA aliquot for adapter ligation, 1.5X bead clean plate C, moving plate A samples • November 9, 2021
Plate B Row A Dilution and Adapter Addition, Plate Reader Plate A • December 18, 2021
Plate B Split, Bead Clean, Plate Read • April 29, 2021
Plate B and C Adapater Ligations • November 27, 2021
Plate Reader - Post 1.5X bead clean plate C and plate A • November 9, 2021
Plate Reader - Raw DNA for Plate A on Plate Reader • November 14, 2021
Pool 1 Blue Pippin Size Selection • January 8, 2022
Pool 2 Blue Pippin Size Selection, QC Pool 1 Size Selected Sample • January 12, 2022
Pool Plate A Pool 1 • December 29, 2021
Pooling Plate C - Pools 4 and 5 • December 16, 2021
Post Digestion Bead Clean • February 2, 2021
Post Digestion Bead Clean and Plate Read • April 27, 2021
Re-run Pool 1 Blue Pippin, Pools 3,4,5 PCR and Index Addition • January 26, 2022
Sample Pooling • August 5, 2021
Sample Pooling • July 8, 2021
Second Attempt Adapter Ligation Dilutions • July 27, 2021
Sorted through all plates and freezer and bead cleaned plate C • November 2, 2021
Summary of all Plate Reader, Qubit, and Tape Station Tests • October 22, 2021
Tape Station Procedure for Digested Samples • February 4, 2021
Tape Station Procedure for Digested Samples (250ng) • March 25, 2021
Tape Station Results Samples 359, 854, 858 • March 30, 2021
Tape Station Results Samples 401, 759, 419 • February 25, 2021
Trouble Shooting with Jon • August 17, 2021

PCR 2

Gel for QAQC Pools 1 and 2 (Tape Station Out of Service) • January 22, 2022
PCR Index Addition, 1.5X Bead Clean, Post Quibit Size Selected Pools 1 and 2 • January 19, 2022

blue pippin 2

Pool 1 Blue Pippin Size Selection • January 8, 2022
Pool 2 Blue Pippin Size Selection, QC Pool 1 Size Selected Sample • January 12, 2022

ddRAD 4

Additional 4 cycles PCR for all pools • February 24, 2022
First Half DNA Extraction • March 9, 2022
First Half DNA Extraction • February 16, 2022
Mailing 5 Libraries • March 14, 2022

size selection 3

Blue Pippin Pools 3,4,5 • January 23, 2022
PCR and Index Addition Pool 1, 1.5X clean pools 1,3,4,5, qubit, gel • February 1, 2022
Re-run Pool 1 Blue Pippin, Pools 3,4,5 PCR and Index Addition • January 26, 2022

troubleshooting 12

DNA Extraction, Digestion on Plate C, Plate Reader on Plate B • November 3, 2021
Ligation Efficiency Test Plate A, Pool Plate B Pool 3 • December 23, 2021
Ligation Efficiency Test, Adapter Ligation, Pool 2 Bead Clean • December 22, 2021
Plate A Dilution for Ligation • December 19, 2021
Plate A and C Dilutions • November 17, 2021
Plate B 100ng DNA aliquot for adapter ligation, 1.5X bead clean plate C, moving plate A samples • November 9, 2021
Plate B and C Adapater Ligations • November 27, 2021
Plate Reader - Post 1.5X bead clean plate C and plate A • November 9, 2021
Plate Reader - Raw DNA for Plate A on Plate Reader • November 14, 2021
Pool Plate A Pool 1 • December 29, 2021
Sorted through all plates and freezer and bead cleaned plate C • November 2, 2021
Summary of all Plate Reader, Qubit, and Tape Station Tests • October 22, 2021