Plate Reader - Post 1.5X bead clean plate C and plate A
Plate Reader for Plates A and C
Date Performed: November 13th, 2021
Standard layout for both plates: 0,0,2.5.2.5,6.25,6.25,12.5,12.5,25,25
Master Mix Plate A: ~60 samples, 12000ul buffer, 120ul enhancer, 120ul dye
Master Mix Plate C: ~80 samples, 16000ul buffer, 160ul enhancer, 160ul dye
Followed standard plate reader protocol, but added 8ul TE buffer to each sample well.
Used Qubit for row H plate, AccuGreen Qubit Kit, Sample number corresponds to well number
| Sample | Run 1 | Run 2 |
|---|---|---|
| S1 | 398.03 | |
| S2 | 106228.07 | |
| 1 | 5.22 | 5.22 |
| 2 | 7.22 | 7.24 |
| 3 | 7.90 | 7.88 |
| 4 | 6.24 | 6.24 |
| 5 | 9.08 | 9.18 |
| 6 | 8.38 | 8.34 |
| 7 | 6.30 | 6.40 |
| 8 | 7.18 | 7.18 |
Next steps: Plate A needs to be restarted, I will quantify raw DNA on plate reader. Plate C needs to be diluted for adapter ligation.
Written on November 9, 2021