Plate C Post Digest Clean and Plate Read

Date Performed: April 27th, 2021

Followed Step 7 From DDRad protocol for post digestion 1.8X bead clean on plate C
https://docs.google.com/document/d/1iWBGgBZuXlVuiEV-A3ecxeyn9ZaY389tAqGZWksjs5Y/edit

Followed Protocol for Plate Reader:
https://docs.google.com/document/d/1iWBGgBZuXlVuiEV-A3ecxeyn9ZaY389tAqGZWksjs5Y/edit

For Plate C made the following master mix:\

• 37 samples plus 12 standards
• 10mL buffer
• 100ul dye
• 100ul enhancer

(200ul buffer per sample + 2ul dye per sample + 2ul enhancer per sample plus error, also include each standard you’re going to do here as a sample)

Standard Setup

• 2 0ng/ul
• 2 2ng/ul
• 2 12ng/ul
• 2 25ng/ul
• 1 50ng/ul
• 1 100ng/ul

Plate Read Result:

Layout Same as Plate Setup

Plate Reader said no DNA present in Plate C, ran a few samples on Qubit to confirm
Samples 887, 760, 422, 769, 403 from plate B for comparison