Plate B Split for Troubleshooting Post Digest

Date Performed: April 29th, 2021

Split Plate B into Plate B and D:

Followed Step 7 From DDRad protocol for post digestion PERFORMED AS 1.5X bead clean
https://docs.google.com/document/d/1iWBGgBZuXlVuiEV-A3ecxeyn9ZaY389tAqGZWksjs5Y/edit\

Maggie did Plate B
Natalie did Plate D\

Notes
For last step of bead clean removed liquid and added final water one row at a time
Moving plate up one space on magnet at least a minute before adding nuclease free water really helped mix\

Followed Protocol for Plate Reader:
https://docs.google.com/document/d/1iWBGgBZuXlVuiEV-A3ecxeyn9ZaY389tAqGZWksjs5Y/edit

For Plates B and D made the following master mix:\

• 74 samples plus 12 standards
• 20mL buffer
• 200ul dye
• 200ul enhancer

Standard Setup

• 2 0ng/ul
• 2 2ng/ul
• 2 12ng/ul
• 2 25ng/ul
• 1 50ng/ul
• 1 100ng/ul

Plate Read Result: