Horseshoe Crab DNA (re)Extraction and Quantification
Summary of all DNA re-extractions and re-gels
Tissue samples selected for re-extraction (42 total): 769, 770, 772, 784, 785, 863, 869, 870, 425, 444, 447, 450, 455, 464
466, 467, 470, 488, 776, 777, 780, 838, 887, 441-443, 445, 446, 448, 449, 452-454, 491, 794, 796, 818, 824, 835, 836, 859, 886
Extractions chosen for re-gel only: 393, 400, 404, 406, 416, 434, 451, 472, 483, 751, 762, 781, 788, 789, 790, 795, 801, 839, 840, 861, 866
DNA Extracted following Version 3 Extraction Protocol
Used same assay procedure as in previous post
Results from re-extractions
| Sample | Run 1 | Run 2 |
|---|---|---|
| 770 | 90.8 | 70.8 |
| 772 | 48.6 | 49.0 |
| 784 | 39.0 | 39.0 |
| 785 | 111 | 112 |
| 863 | 45.2 | 44.2 |
| 869 | 55.0 | 53.8 |
| 870 | 45.2 | 48.2 |
| 769 | 30.4 | 30.6 |
| 425 | 95.0 | 95.4 |
| 444 | 77.2 | 78.4 |
| 447 | 59.4 | 60.0 |
| 450 | 56.0 | 56.8 |
| 455 | 49.8 | 50.2 |
| 464 | 29.8 | 30.6 |
| 466 | 71.4 | 72.4 |
| 467 | 57.4 | 58.0 |
| 470 | 55.6 | 56.2 |
| 488 | 50.6 | 51.0 |
| 776 | 46.0 | 46.6 |
| 777 | 55.4 | 55.8 |
| 780 | 50.8 | 50.8 |
| 838 | 52.8 | 54.0 |
| 887 | 65.6 | 66.4 |
| 441 | 82.4 | 83.2 |
| 442 | 76.0 | 75.2 |
| 443 | 125 | 125 |
| 445 | 72.0 | 72.2 |
| 446 | 71.6 | 72.2 |
| 448 | 124 | 126 |
| 449 | 103 | 103 |
| 452 | 79.0 | 79.8 |
| 453 | 100 | 101 |
| 454 | 87.0 | 88.2 |
| 491 | 93.0 | 92.6 |
| 794 | 96.6 | 98.0 |
| 796 | 69.2 | 69.6 |
| 818 | 59.8 | 60.8 |
| 824 | 65.8 | 65.6 |
| 835 | 104 | 105 |
| 836 | 76.0 | 76.2 |
| 859 | 78.6 | 79.8 |
| 886 | 98.4 | 99.6 |
Gels from re-extractions and re-gels:
Re-gels are in this top image
Written on January 11, 2021