Horseshoe Crab DNA Extraction and Quantification
Horseshoe Crab DNA Extraction and Quantification - Qubit Assay Broad Range dsDNA
Date of Extraction: November 17th, 2020
Date of Assay: November 17th, 2020
Tissue samples extracted: 414-416, 419-422, 424, 497, 498, 787-790, 802-804, 806, 807, 810
Tissue samples quantified: 414-416, 419-422, 424, 497, 498, 787-790, 802-804, 806, 807, 810
Notes: only added 7ul of DNA to strip tubes because 1ul of DNA added directly to axygen tubes for assay. Accidentally added 10ul sample 371 to strip tube.
DNA Extracted following Version 3 Extraction Protocol
Used same assay procedure as in previous post
| Sample | Run 1 | Run 2 |
|---|---|---|
| S1 | 189.57 | |
| S2 | 20367.76 | |
| 414 | 74.4 | 74.2 |
| 415 | 69.4 | 71.2 |
| 416 | 74.6 | 74.8 |
| 419 | 74.6 | 75.0 |
| 420 | 59.6 | 60.0 |
| 421 | 66.2 | 66.6 |
| 422 | 70.8 | 71.2 |
| 424 | 61.8 | 62.6 |
| 497 | 74.4 | 75.4 |
| 498 | 63.6 | 64.0 |
| 787 | 75.4 | 75.4 |
| 788 | 81.6 | 81.6 |
| 789 | 76.4 | 77.4 |
| 790 | 59.4 | 60.2 |
| 802 | 71.2 | 71.8 |
| 803 | 63.8 | 64.4 |
| 804 | 78.0 | 78.8 |
| 806 | 60.0 | 60.4 |
| 807 | 61.4 | 61.8 |
| 810 | 84.8 | 85.2 |
Written on November 17, 2020