Gel on pools 1 and 2

Date Performed: January 22nd, 2022

Tape station not working so using gel instead for fragment size check

Step 1 - Amplify 1ul of post PCR Pools

• Pool 1
  • 2 ul sample
  • 10ul Kapa Hifi
  • .5 ul 502 primer
  • .5 ul 702 primer
  • 7ul nuclease free water *Pool 2
  • 1 ul sample
  • 10ul Kapa Hifi
  • .5 ul 505 primer
  • .5 ul 705 primer
  • 8ul nuclease free water Ran 12 cycle PCR. Post PCR 1.5X bead clean (20ul*1.5=30ul beads), re-eluted in 20ul 10mMtris HCL

Ran second round of Qubit on initial PCR pools because of weird results previously