DNA Extraction, Digest, Plate Reader

Date Performed: 11/3/2021

1. DNA Extraction results can be found here: https://njameral.github.io/Ameral_Lab_Notebook/All-Gels-2021-samples/

2. Plate B - Plate reader
   Followed protocol, but added 8ul TE buffer for even volumes in all wells

3. Digestion plate C - PST and EcoR1 - did not leave beads in for digestion. I have been having issue with having enough digestion MM. I only had enough for 71 samples, but I made enough for 80 samples.
   I had 74 samples to process so this means I would need to make 12 extra to have enough. Not sure how this makes sense. Calculations below:
   Number of samples = 74
   Number of error = 6
   Total preps = 80
   1ul Pst/sample = 80 ul Pst1
   1ul EcoR1/sample = 80 ul Pst1
   80ul buffer/samp = 640 ul buffer
   Total volume MM = 800ul
   800ul/10ul per sample = preps for 80 samples