Mailing 5 Libraries
Mailed out first batch to NovoGene
Date Performed: March 14th, 2022
First Half DNA Extraction
DNA Extraction 2021 Samples
Date Performed: March 9th, 2022
Additional 4 cycles PCR for all pools
Ran 4 more cycles PCR for all pools (1-5) due to low DNA concentration
Date Performed: February 24th, 2022
First Half DNA Extraction
DNA Extraction 2021 Samples
Date Performed: February 16th, 2022
PCR and Index Addition Pool 1, 1.5X clean pools 1,3,4,5, qubit, gel
PCR for Pool 1, 1.5X clean, qubit, gel pools 1,3,4,5
Date Performed: February 1st, 2022
Re-run Pool 1 Blue Pippin, Pools 3,4,5 PCR and Index Addition
Run Pool 1 Size Selection with Tight Setting, PCR 3,4,5
Date Performed: January 26th, 2022
Blue Pippin Pools 3,4,5
Size Selection Pools 3,4,5
Date Performed: January 23rd, 2022
Gel for QAQC Pools 1 and 2 (Tape Station Out of Service)
Gel on pools 1 and 2
Date Performed: January 22nd, 2022
PCR Index Addition, 1.5X Bead Clean, Post Quibit Size Selected Pools 1 and 2
Pools 1 and 2 PCR, 1.5X Clean, and 2X Qubit
Date Performed: January 19th, 2022
Pool 2 Blue Pippin Size Selection, QC Pool 1 Size Selected Sample
Pool 2 Plate B Size Selection and Pool 1 QC
Date Performed: January 12th, 2022
Pool 1 Blue Pippin Size Selection
Pool 1 Plate A Size Selection
Date Performed: January 8th, 2022
Pool Plate A Pool 1
Pooling Plate A Pool 1
Date Performed: December 29th, 2021
Ligation Efficiency Test Plate A, Pool Plate B Pool 3
Ligation Efficiency test for Plate A, Pooling Pool 3 from Plate B
Date Performed: December 23rd, 2021
Ligation Efficiency Test, Adapter Ligation, Pool 2 Bead Clean
Ligation Efficiency test for Plate B Row A, Adapter Ligation for Plate A, Pooling Pool 2 from Plate B
Date Performed: December 22nd, 2021
Plate A Dilution for Ligation
Plate A Dilution for 100ng DNA Adapter Ligation
Date Performed: December 19th, 2021
Plate B Row A Dilution and Adapter Addition, Plate Reader Plate A
Plate B Row A dilution and adapters, plate reader Plate A
Date Performed: December 18th, 2021
Pooling Plate C - Pools 4 and 5
Pooling Plate C then doing 2 1.5X cleans
Date Performed: December 16th, 2021
Ligation Test Plates B and C, Bead Clean Plate A
Ligation Tests for Plates B and C, 1.5X Bead Clean Plate A
Date Performed: December 1st, 2021
Plate A 1X Bead Clean and Digestion
Plate A 1X Bead Clean and Digestion
Date Performed: November 30th, 2021
Plate B and C Adapater Ligations
Plate B and C adapter Ligations
Date Performed: November 27th, 2021
Plate A and C Dilutions
Plate C Dilution for 100ng DNA Adapter Ligation, Plate A 500ng for DNA digest
Date Performed: November 17th, 2021
Plate Reader - Raw DNA for Plate A on Plate Reader
Plate A Plate Reads - Pre-Dilution
Date Performed: November 14th, 2021
Plate Reader - Post 1.5X bead clean plate C and plate A
Plate Reader for Plates A and C
Date Performed: November 13th, 2021
Plate B 100ng DNA aliquot for adapter ligation, 1.5X bead clean plate C, moving plate A samples
Plate B Aliquot, Plate C 1.5X bead clean, plate A transfer to new plate
Dates Performed: November 9th and 11th, 2021
DNA Extraction, Digestion on Plate C, Plate Reader on Plate B
DNA Extraction, Digest, Plate Reader
Date Performed: 11/3/2021
Sorted through all plates and freezer and bead cleaned plate C
Bead Cleaning and Organizing
Summary of all Plate Reader, Qubit, and Tape Station Tests
Summary of Tests
Digestion (Testing) with Plate B
1X Bead Clean, Qubit, Digestion
Dates Performed: September 2nd, 9th, 14th
Gel Images with Average DNA ng/ul for all 2021 Extractions
Gel Images for all 2021 Samples
1x Bead Cleans, Digestions, 1.5X Bead Clean and Post Digest Quant for Plate A
Bead Cleans, Digestions, Quantifications
Dates Performed: August 30th, September 14th
Plate Aliquots
Aliquotting Out Samples for Digestion (500ng DNA per 50ul)
Date Performed: August 26th, 2021
Comparison Run of Qubit and Plate Reader
Qubit and Plate Reader
Date Performed: August 19th
Trouble Shooting with Jon
Bead cleans, pooling, Qubit
Date Performed: August 17th
Sample Pooling
Two 1.5X bead cleans and plate pooling with Qubit in between
Date Performed: August 5th Plates B and C
Ligation Efficiency Testing
PCR Test and Gel for Ligation Test
Date Performed: August 3rd
Performed on Plates B, C, D, E
Second Attempt Adapter Ligation Dilutions
Diluted Plates for Adapter Ligation and Qubit Testing
Dates Performed: July 27th and 29th and August 3rd
Horseshoe Crab DNA Extraction and Quantification 2021 Samples
Horseshoe Crab DNA Extraction and Quantification - Qubit Assay Broad Range dsDNA
PCR, Index Addition, 1X Bead Clean, Qubit
Index additions, cleaning, quantification
Date Performed: July 22nd
Blue Pippin Size Selection
Size Selection of 525-600 bps on all pools
Dates Performed: July 20th Pools 1,2,3,4,5 July 22nd Pool 2
Sample Pooling
Two 1.5X bead cleans and plate pooling
Dates Performed: July 8th (Plates) July 13th (1st Bead Clean Plate B) July 15th (2nd Bead Clean Plate B, C, D, E)
Ligation Efficiency Testing
PCR Test and Gel for Ligation Test
Dates Performed: July 6th (Plates A,B,D) July 8th (Plates C,E)
Annealing Adapters and Adapter Ligation
Adapter Annealing and Adapter Ligation
Dates Performed: June 29th, 30th, July 1st and 6th
Bead Cleans and Plate Reader Quantification
Post Digest Bead Cleans and Plate Reads
Dates Performed: June 22nd and 24th
Plate Aliquots
DNA Dilutions, 1X Bead Cleans, Digestions plates A-E (350ng DNA per 50ul)
Dates Performed: June 3rd, 8th, 9th, 19th, 21st 2021
Digestion Testing
Testing Digestion on 12 Samples
Date Performed: May 19th and 20th, 2021
Digestion Testing
Testing Digestion on 12 Samples
Date Performed: May 16th and 17th, 2021
Dilution Testing
Testing Pre and Post Dilution Qubit Values
Date Performed: May 11th, 2021
Bead Testing
Bead Testing on Old and New Beads
Date Performed: May 6th, 2021
Plate B Split, Bead Clean, Plate Read
Plate B Split for Troubleshooting Post Digest
Date Performed: April 29th, 2021
Post Digestion Bead Clean and Plate Read
Plate C Post Digest Clean and Plate Read
Date Performed: April 27th, 2021
Bead Cleans and Digestions for Plates A, B, C
Bead Cleans and Digestions
Dates Performed: April 15th, 20th, 22nd 2021
Plate Aliquots
Aliquotting Out Samples for Digestion (350ng DNA per 50ul)
Dates Performed: April 6th and 8th, 2021
Tape Station Results Samples 359, 854, 858
Tape Station Reports - Locus Estimation (250ng DNA)
Tape Station Procedure for Digested Samples (250ng)
Tape Station
Dates Performed: March 25th
Digestion Testing with 250ng DNA
Enzyme Reaction Testing with 250ng DNA
Date Performed: March 23rd, 2021\
Digestion Testing with PST/MSP
Enzyme Reaction Testing with Selected Enzymes and Bead Clean
Date Performed: March 18th, 2021 Samples used: 352, 369, 431, 439, 487, 785, 831, 865 (randomly selected)
Tape Station Results Samples 401, 759, 419
Tape Station Reports - Locus Estimation (500ng DNA)
Tape Station Procedure for Digested Samples
Tape Station
Dates Performed: February 4th and 16th, 2021 Samples run: 401 and 759 on February 4th, 419 run on February 16th
Post Digestion Bead Clean
1.8X Bead Clean on Digested Samples
Date Performed: February 2nd, 2021
Digestion Testing
Enzyme Reaction Testing
Date Performed: January 28th, 2021
Digest Prep - First Bead Clean for Enzyme Testing
Bead Clean for Enzyme Testing
Samples selected for test: 401, 419, 759
Date Performed: January 26th, 2021
These samples were chosen based on location, date collected, sex, and DNA concentration/gel quality
Horseshoe Crab DNA Third Extraction Attempt 464, 769
Horseshoe Crab DNA Extraction, Quantification, and Gel
Date of Extraction: January 26th, 2021
Date of Assay: January 26th, 2021
Horseshoe Crab Agarose (Re)Gel Results for 464 and 769
Horseshoe Crab Agarose Gel
Date Performed: January 21st, 2021
Samples run: 464, 464-2, 769, 769-2 (all were re-gels, wanted to double check quality before doing re-extraction)
Horseshoe Crab DNA (re)Extraction and Quantification
Summary of all DNA re-extractions and re-gels
Horseshoe Crab Agarose Gel Results from Re-Extraction
Horseshoe Crab Agarose Gel
Date Performed: January 5th, 2021
Samples run: 769, 770, 772, 784, 785, 863, 869, 870, 393, 400, 404, 406, 416, 434, 451, 472, 483, 751, 762, 781, 788, 789, 790, 795, 801, 839, 840, 861, 866
Horseshoe Crab Agarose Gel Results from Re-Extraction
Horseshoe Crab Agarose Gel
Date Performed: December 29th, 2020
Samples run: 425, 444, 447, 450, 455, 464, 466, 467, 470, 488, 776, 777, 780, 838, 887
Horseshoe Crab DNA (re)Extraction and Quantification
Horseshoe Crab DNA (re)Extraction and Quantification - Qubit Assay Broad Range dsDNA
Date of Extraction: December 29th, 2020
Date of Assay: January 5th, 2021
Horseshoe Crab Agarose Gel Results from Re-Extraction
Horseshoe Crab Agarose Gel
Date Performed: December 22nd, 2020
Samples run: 441-443, 445, 446, 448, 449, 452-454, 491, 794, 796, 818, 824, 835, 836, 859, 886
Horseshoe Crab DNA (re)Extraction and Quantification
Horseshoe Crab DNA (re)Extraction and Quantification - Qubit Assay Broad Range dsDNA
Date of Extraction: December 22nd, 2020
Date of Assay: December 22nd, 2020
Horseshoe Crab DNA (re)Extraction and Quantification
Horseshoe Crab DNA (re)Extraction and Quantification - Qubit Assay Broad Range dsDNA
Date of Extraction: December 10th, 2020
Date of Assay: December 10th, 2020
Gel Images with Average DNA ng/ul for all 2020 Extractions
Gel Images for all Initial Extractions of 2020 Samples
Horseshoe Crab Agarose Gel Results
Horseshoe Crab Agarose Gel
Date Performed: December 3rd, 2020
Samples run: 874-881, 905, 097, 462-464, 489, 490, 845-852, 854, 855
Horseshoe Crab DNA Quantification
Horseshoe Crab DNA Extraction - Qubit Assay Broad Range dsDNA
Date of Assay: December 3rd, 2020
Horseshoe Crab Agarose Gel Results
Horseshoe Crab Agarose Gel
Date Performed: December 1st, 2020
Samples run: 804, 806, 807, 810, 791-793, 795, 797, 814, 815, 819, 821-823, 832-834, 837, 839, 840, 898, 843, 844, 481-485, 882-885, 871-873
Horseshoe Crab DNA Extraction and Quantification
Horseshoe Crab DNA Extraction and Quantification - Qubit Assay Broad Range dsDNA
Date of Extraction: December 1st, 2020
Date of Assay: December 1st, 2020
Horseshoe Crab Agarose Gel Results
Horseshoe Crab Agarose Gel
Date Performed: November 24th, 2020
Samples run: 414-416, 419, 420, 421, 422, 424, 497, 498, 787-790, 802, 803, 371, 372, 073
Horseshoe Crab DNA Extraction and Quantification
Horseshoe Crab DNA Extraction and Quantification - Qubit Assay Broad Range dsDNA
Date of Extraction: November 24th, 2020
Date of Assay: November 24th, 2020
Horseshoe Crab DNA Extraction and Quantification
Horseshoe Crab DNA Extraction - Qubit Assay Broad Range dsDNA
Date of Extraction: November 19th, 2020
Horseshoe Crab DNA Extraction and Quantification
Horseshoe Crab DNA Extraction and Quantification - Qubit Assay Broad Range dsDNA
Date of Extraction: November 17th, 2020
Date of Assay: November 17th, 2020
Horseshoe Crab Agarose Gel Results
Horseshoe Crab Agarose Gel
Date Performed: November 14th, 2020
Samples run: 387-388, 456-461, 377-380, 383-386, 389-404, 406-409
Horseshoe Crab DNA Extraction and Quantification
Horseshoe Crab DNA Extraction and Quantification - Qubit Assay Broad Range dsDNA
Date of Extraction: November 14th, 2020
Date of Assay: November 14th, 2020
Horseshoe Crab Agarose Gel Results
Horseshoe Crab Agarose Gel
Date Performed: November 12th, 2020
Samples run: 412,413, 428-430, 766, 373-376, 767, 768, 811-813, 826-831
Horseshoe Crab DNA Extraction and Quantification
Horseshoe Crab DNA Extraction and Quantification - Qubit Assay Broad Range dsDNA
Date of Extraction: November 12th, 2020
Date of Assay: November 12th, 2020
Horseshoe Crab DNA Extraction and Quantification
Horseshoe Crab DNA Extraction and Quantification - Qubit Assay Broad Range dsDNA
Date of Extraction: November 10th, 2020
Date of Assay: November 10th, 2020
Horseshoe Crab Agarose Gel Results
Horseshoe Crab Agarose Gel
Date Performed: November 5th, 2020 Samples run: 356-364, 366-369, 761, 411, 351-355
Horseshoe Crab DNA Extraction and Quantification
Horseshoe Crab DNA Extraction and Quantification - Qubit Assay Broad Range dsDNA
Date of Extraction: November 5th, 2020
Date of Assay: November 5th, 2020
Horseshoe Crab DNA Extraction and Quantification
Horseshoe Crab DNA Extraction and Quantification - Qubit Assay Broad Range dsDNA
Date of Extraction: November 3rd, 2020
Date of Assay: November 3rd, 2020
Horseshoe Crab Agarose Gel Results
Horseshoe Crab Agarose Gel
Date Performed: October 29th, 2020
Samples run: 777-780,783-786, 769-776, 477-480, 861-870, 471-476
Horseshoe Crab DNA Extraction
Horseshoe Crab DNA Extraction with Zymo Quick DNA Miniprep Plus kit
Date Performed: October 22nd, 2020
Tissue samples processed: 861-870, 471-480 (20 samples)
Horseshoe Crab DNA Quantification
Horseshoe Crab DNA Quantification - Qubit Assay Broad Range dsDNA
Date Performed: October 22nd, 2020
Tissue samples quantified: 861-870, 471-480
Horseshoe Crab DNA Extraction
Horseshoe Crab DNA Extraction with Zymo Quick DNA Miniprep Plus kit
Date Performed: October 20th, 2020
Tissue samples processed: 769-780, 783-786 (16 samples)
Horseshoe Crab DNA Quantification
Horseshoe Crab DNA Quantification - Qubit Assay Broad Range dsDNA
Date Performed: October 20th, 2020
Tissue samples quantified: 769-780, 783-786 (16 samples)
Horseshoe Crab Agarose Gel Results
Horseshoe Crab Agarose Gel
Date Performed: October 15th, 2020
Samples run: 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 486, 487, 751, 752, 753, 754, 755, 756, 757, 758, 759, 760
Horseshoe Crab DNA Extraction
Horseshoe Crab DNA Extraction with Zymo Quick DNA Miniprep Plus kit
Date Performed: October 13th, 2020
Tissue samples processed: 431-440,486,487
Horseshoe Crab DNA Quantification
Horseshoe Crab DNA Quantification - Qubit Assay Broad Range dsDNA
Date Performed: October 13th, 2020
Tissue samples quantified: 431-440, 486, 487
Horseshoe Crab Agarose Gel Results
Horseshoe Crab Agarose Gel
Date Performed: October 8th, 2020
Samples run: 465, 466, 467, 468, 469, 470, 841, 842, 801, 488
Horseshoe Crab DNA Extraction
Horseshoe Crab DNA Extraction with Zymo Quick DNA Miniprep Plus kit
Date Performed: October 8th, 2020
Tissue samples processed: 751-760
Horseshoe Crab DNA Quantification
Horseshoe Crab DNA Quantification - Qubit Assay Broad Range dsDNA
Date Performed: October 8th, 2020
Tissue samples quantified: 751-760
Horseshoe Crab DNA Extraction
Horseshoe Crab DNA Extraction with Zymo Quick DNA Miniprep Plus kit
Date Performed: October 6th, 2020
Tissue samples processed: 465, 466, 467, 468, 469, 470, 841, 842, 488, 801
Horseshoe Crab DNA Quantification
Horseshoe Crab DNA Quantification - Qubit Assay Broad Range dsDNA
Date Performed: October 6th, 2020
Tissue samples quantified: 465, 466, 467, 468, 469, 470, 841, 842, 488, 801
Horseshoe Crab Agarose Gel Results
Horseshoe Crab Agarose Gel
Date Performed: September 16th, 2020
Samples run: 491, 445, 444-1, 425, 818, 824, 887, 794, 796, 835, 836, 838, 886, 447, 448, 449, 450, 452, 453, 454, 455
Horseshoe Crab Agarose Gel Protocol
Horseshoe Crab Agarose Gel Protocol - Instructions Only
Derived from Maggie’s Gel Protocol
Horseshoe Crab Agarose Gel Results
Horseshoe Crab Agarose Gel
Date Performed: September 12th, 2020
Samples run: 441, 442, 443, 444-2, 446, 859
Horseshoe Crab DNA Extraction - Version 2
Horseshoe Crab DNA Extraction with Zymo Quick DNA Miniprep Plus kit
Date Performed: September 12th, 2020
Tissue samples processed: 447, 448, 449, 450, 452, 453, 454, 455
Horseshoe Crab DNA Quantification
Horseshoe Crab DNA Quantification - Qubit Assay Broad Range dsDNA
Date Performed: September 12th, 2020 Tissue samples quantified: 447, 448, 449, 450, 452, 453, 454, 455
Horseshoe Crab DNA Extraction - Version 3
Horseshoe Crab DNA Extraction with Zymo Quick DNA Miniprep Plus kit - Protocol Only
Horseshoe Crab DNA Extraction
Horseshoe Crab DNA Extraction with Zymo Quick DNA Miniprep Plus kit
Date Performed: September 11th, 2020
Tissue samples processed: 794, 796, 835, 836, 838, 886
Horseshoe Crab DNA Quantification
Horseshoe Crab DNA Quantification - Qubit Assay Broad Range dsDNA
Date Performed: September 11th, 2020 SECOND ROUND
Tissue samples quantified: 794, 796, 835, 836, 838, 886
Horseshoe Crab DNA Quantification
Horseshoe Crab DNA Quantification - Qubit Assay Broad Range dsDNA
Date Performed: September 11th, 2020
Tissue samples quantified: 441, 442, 443, 444-2, 446, 859
Horseshoe Crab DNA Extraction
Horseshoe Crab DNA Extraction with Zymo Quick DNA Miniprep Plus kit
Date Performed: September 2nd, 2020
Tissue samples processed: 414, 442, 443, 444-2(duplicate), 446, 859
Horseshoe Crab DNA Quantification
Horseshoe Crab DNA Quantification - Qubit Assay Broad Range dsDNA
Date Performed: September 2nd, 2020
Tissue samples quantified: 425, 444, 445, 491, 818, 824, 887
Horseshoe Crab DNA Extraction
Horseshoe Crab DNA Extraction with Zymo Quick DNA Miniprep Plus kit
Date Performed: August 27th, 2020
Tissue samples processed: 818, 887, 824, 425
Horseshoe Crab DNA Extraction
Horseshoe Crab DNA Extraction with Zymo Quick DNA Miniprep Plus kit
Date Performed: August 21st, 2020
Tissue samples processed: 444, 445, 491